Ultradeep bisulfite sequencing analysis of DNA methylation patterns in multiple gene promoters by 454 sequencing.

Abstract excerpt

We developed a novel approach for conducting multisample, multigene, ultradeep bisulfite sequencing analysis of DNA methylation patterns in clinical samples. A massively parallel sequencing-by-synthesis method (454 sequencing) was used to directly sequence >100 bisulfite PCR products in a single sequencing run without subcloning. We showed the utility, robustness, and superiority of this approach by analyzing...

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• CpG Islands
• DNA Methylation
• Genome, Human
• Leukemia, Lymphocytic, Chronic, B-Cell
• Lymphoma, Non-Hodgkin