Isolation of temperature-sensitive cell cycle mutants from mouse FM3A cells. Characterization of mutants with special reference to DNA replication.

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Article

Isolation of temperature-sensitive cell cycle mutants from mouse FM3A cells. Characterization of mutants with special reference to DNA replication.

The Journal of biological chemistry - 5 Jan 1990

Eki T, Enomoto T, Miyajima A, Miyazawa H, Murakami Y, Hanaoka F, Yamada M, Ui M

Abstract excerpt

A large number of mutants that are temperature sensitive (ts) for growth have been isolated from mouse mammary carcinoma FM3A cells by an improved selection method consisting of cell synchronization and short exposures to restrictive temperature. The improved method increased the efficiency of isolating DNA ts mutants, which showed a rapid decrease in DNA-synthesizing ability after temperature shift-up. Sixteen...

Topics

Animals
Autoradiography
Cell Cycle
Cell Separation
Cell-Free System
DNA
DNA Replication
Flow Cytometry
Interphase
Mammary Neoplasms, Experimental
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Phenotype

Polyomavirus

Temperature

Tumor Cells, Cultured

Virus Replication

Source

Open in PubMed

Identifiers

PMID: 2152921

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2152921-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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