Article
Characterization of DNA synthesis at a restrictive temperature in the temperature-sensitive mutants, tsFT5 cells, that belong to the complementation group of ts85 cells containing a thermolabile ubiquitin-activating enzyme E1. Involvement of the ubiquitin-conjugating system in DNA replication.
The Journal of biological chemistry - 5 Aug 1993
Mori M, Eki T, Takahashi-Kudo M, Hanaoka F, Ui M, Enomoto T
Abstract excerpt
A temperature-sensitive mutant defective in DNA replication, tsFT5, has been isolated from the mouse mammary carcinoma cell line FM3A. DNA synthesis in tsFT5 cells at a restrictive temperature (39 degrees C) has been characterized in detail. Incorporation of [3H]thymidine decreased rapidly after...
Topics
- Animals
- Autoradiography
- Cell Cycle
- Cell Division
- DNA Replication
- Genetic Complementation Test
- Histones
- Ligases
- Mice
- Mice, Inbred C3H
- Mutation
- Phosphorylation
- Temperature
- Tumor Cells, Cultured