Article
Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
Cell structure and function - 1 Apr 1992
Ayusawa D, Kaneda S, Itoh Y, Yasuda H, Murakami Y, Sugasawa K, Hanaoka F, Seno T
Abstract excerpt
A temperature-sensitive growth mutant tsFS20 isolated from mouse FM3A cells was identified as a mutant with thermolabile ubiquitin-activating enzyme E1 by transfection with a full-length cDNA encoding the human E1 enzyme and cell-cell hybridization with an authentic E1 mutant ts85 previously isolated from FM3A cells. The resulting transformants produced thermoresistant E1 activity. Upon shift-up of temperature,...
Topics
- Animals
- Cell Cycle
- Cell Line
- Cloning, Molecular
- DNA
- Genetic Complementation Test
- Hot Temperature
- Humans
- Ligases
- Mice
- Molecular Sequence Data