Conditional resistance to thymineless death predominantly selects DNA synthesis-deficient mutants of mammalian cells.

doi:10.1016/0027-5107(93)90133-z

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Article

Conditional resistance to thymineless death predominantly selects DNA synthesis-deficient mutants of mammalian cells.

Mutation research - 1 Sept 1993

Yamao F, Nagai Y, Kaneda S, Yoshida S, Seno T

Abstract excerpt

Temperature-sensitive growth mutants of the mouse mammary carcinoma cell line FM3A were isolated by selecting survivors of thymidylate starvation for a limited time at the restrictive temperature (39.5 degrees C). Nineteen lines of independent isolates were established and all were found to be de...

Topics

Animals
DNA
Electrophoresis, Gel, Pulsed-Field
Genetic Complementation Test
Mammary Neoplasms, Experimental
Mice
Mutation
Temperature
Thymine
Tumor Cells, Cultured

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