Article
Characterization of DNA synthesis and DNA-dependent ATPase activity at a restrictive temperature in temperature-sensitive tsFT848 cells with thermolabile DNA helicase B.
Molecular and cellular biology - 1 Jan 1995
Seki M, Kohda T, Yano T, Tada S, Yanagisawa J, Eki T, Ui M, Enomoto T
Abstract excerpt
A temperature-sensitive mutant defective in DNA replication, tsFT848, was isolated from the mouse mammary carcinoma cell line FM3A. In mutant cells, the DNA-dependent ATPase activity of DNA helicase B, which is a major DNA-dependent ATPase in wild-type cells, decreased at the nonpermissive temperature of 39 degrees C. DNA synthesis in tsFT848 cells at the nonpermissive temperature was analyzed in detail. DNA...
Topics
- Animals
- Autoradiography
- Cell Line
- DNA
- DNA Helicases
- DNA Replication
- Hot Temperature
- In Vitro Techniques
- Mice
- Mice, Inbred C3H
- Mutation