Article
A point mutation in C-terminal region of cdc2 kinase causes a G2-phase arrest in a mouse temperature-sensitive FM3A cell mutant.
Cell structure and function - 1 Feb 1991
Yasuda H, Kamijo M, Honda R, Nakamura M, Hanaoka F, Ohba Y
Abstract excerpt
A mouse temperature-sensitive mutant for cell growth, tsFT210, was characterized. More than 90% of the mutant cells were arrested at the G2 phase at the nonpermissive temperature (39 degrees C). In this mutant, the activity of cdc2 kinase did not increase at the nonpermissive temperature (39 degrees C) but did increase at the permissive temperature (33 degrees C) at the G2/M phase in the cell cycle. The in vitro...
Topics
- Amino Acid Sequence
- Animals
- Aphidicolin
- Base Sequence
- Blotting, Western
- CDC2 Protein Kinase
- Diterpenes
- G2 Phase
- Mice
- Molecular Sequence Data
- Mutation