Article
Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function.
Genes & development - 1 Oct 1990
Liao X L, Kretzner L, Seraphin B, Rosbash M
Abstract excerpt
To study the contribution of the large, 568-nucleotide yeast (Saccharomyces cerevisiae) U1 snRNA to pre-mRNA splicing, we generated mutations in two regions of the molecule and introduced each mutant gene back into yeast as the sole copy of the U1 snRNA gene. We mutagenized the "A loop," a subregion highly conserved in primary sequence in all U1 snRNA molecules analyzed to date. We also mutagenized a portion of...
Topics
- Base Sequence
- Fungal Proteins
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Phenotype
- Plasmids
- RNA Splicing
- RNA, Fungal
- RNA, Messenger
- RNA, Small Nuclear