Construction of an in vivo-regulated U6 snRNA transcription unit as a tool to study U6 function.

Article

Construction of an in vivo-regulated U6 snRNA transcription unit as a tool to study U6 function.

RNA (New York, N.Y.) - 1 Feb 1998

Luukkonen B G, Séraphin B

Abstract excerpt

U6 snRNA is the only spliceosomal snRNA transcribed by RNA polymerase III in yeast. We have constructed a regulated U6 snRNA transcription unit by introducing the binding site for the Escherichia coli lacI repressor protein in the U6 snRNA promoter. GAL-induced expression of lacI protein led to a...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
DNA, Recombinant
Escherichia coli
Escherichia coli Proteins
Genes, Fungal
Lac Repressors
Mutation
RNA Splicing
RNA, Small Nuclear
Repressor Proteins
Saccharomyces cerevisiae
Transcription, Genetic

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