Article
Features of the formate dehydrogenase mRNA necessary for decoding of the UGA codon as selenocysteine.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Zinoni F, Heider J, Böck A
Abstract excerpt
The fdhF gene encoding the 80-kDa selenopolypeptide subunit of formate dehydrogenase H from Escherichia coli contains an in-frame TGA codon at amino acid position 140, which encodes selenocysteine. We have analyzed how this UGA "sense codon" is discriminated from a UGA codon signaling polypeptide chain termination. Deletions were introduced from the 3' side into the fdhF gene and the truncated 5' segments were...
Topics
- Aldehyde Oxidoreductases
- Base Sequence
- Cloning, Molecular
- Codon
- Cysteine
- DNA, Recombinant
- Escherichia coli
- Formate Dehydrogenases
- Gene Expression
- Genotype
- Kinetics