Article
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. II. Evidence that a mRNA stem-loop structure is essential for decoding opal (UGA) as selenocysteine.
The Journal of biological chemistry - 25 Nov 1991
Berg B L, Baron C, Stewart V
Abstract excerpt
fdnG, encoding the selenopeptide of Escherichia coli formate dehydrogenase-N, contains an in-frame opal (UGA) codon at amino acid position 196 that directs selenocysteine incorporation. We have identified sequences that contribute to the mRNA context required for decoding this UGA as selenocystei...
Topics
- Base Composition
- Base Sequence
- Cloning, Molecular
- Codon
- Cysteine
- Enzyme Induction
- Escherichia coli
- Formate Dehydrogenases
- Genotype
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nitrates
- Nucleic Acid Conformation
- Oligodeoxyribonucleotides
- Organoselenium Compounds
- Plasmids
- RNA, Messenger
- Selenocysteine