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Article

Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli.

Journal of bacteriology - 1 May 2012

Ohtake Kazumasa, Sato Aya, Mukai Takahito, Hino Nobumasa, Yokoyama Shigeyuki, Sakamoto Kensaku

Abstract excerpt

We previously reassigned the amber UAG stop triplet as a sense codon in Escherichia coli by expressing a UAG-decoding tRNA and knocking out the prfA gene, encoding release factor 1. UAG triplets were left at the ends of about 300 genes in the genome. In the present study, we showed that the detrimental effect of UAG reassignment could be alleviated by increasing the efficiency of UAG translation instead of...

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Identifiers

PMID: 22427623
DOI: 10.1128/JB.00195-12
PMCID: PMC3347192

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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