Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome.

doi:10.1101/gad.1871910

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Article

Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome.

Genes & development - 1 Jan 2010

Saini Adesh K, Nanda Jagpreet S, Lorsch Jon R, Hinnebusch Alan G

Abstract excerpt

eIF1A is the eukaryotic ortholog of bacterial translation initiation factor IF1, but contains a helical domain and long unstructured N-terminal tail (NTT) and C-terminal tail (CTT) absent in IF1. Here, we identify elements in these accessory regions of eIF1A with dual functions in binding methionyl initiator tRNA (Met-tRNA(i)(Met)) to the ribosome and in selecting AUG codons. A pair of repeats in the eIF1A CTT,...

Topics

Amino Acid Sequence
Codon, Initiator
Enhancer Elements, Genetic
Eukaryotic Initiation Factor-1
Gene Expression Regulation, Fungal
Models, Molecular
Molecular Sequence Data
Mutation
Phenotype
Protein Binding

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Structure, Tertiary

RNA, Transfer, Met

Ribosome Subunits, Small, Eukaryotic

Ribosomes

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Sequence Alignment

Source

Open in PubMed Open full text

Identifiers

PMID: 20048003
DOI: 10.1101/gad.1871910
PMCID: PMC2802195

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Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome.PMID 20048003Read ↗

The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo.Semantic proximity Read ↗

Eukaryotic translation initiation factor eIF5 promotes the accuracy of start codon recognition by regulating Pi release and conformational transitions of the preinitiation complex.Semantic proximity Read ↗

Translation initiation factor 2gamma mutant alters start codon selection independent of Met-tRNA binding.Semantic proximity Read ↗

Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo.Semantic proximity Read ↗

The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2β.Semantic proximity Read ↗

Functional elements in initiation factors 1, 1A, and 2β discriminate against poor AUG context and non-AUG start codons.Semantic proximity Read ↗

Conserved residues in yeast initiator tRNA calibrate initiation accuracy by regulating preinitiation complex stability at the start codon.Semantic proximity Read ↗

Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex.Semantic proximity Read ↗

β-Hairpin loop of eukaryotic initiation factor 1 (eIF1) mediates 40 S ribosome binding to regulate initiator tRNA(Met) recruitment and accuracy of AUG selection in vivo.Semantic proximity Read ↗

IF2 and unique features of initiator tRNAfMet help establish the translational reading frame.Semantic proximity Read ↗

Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo.Semantic proximity Read ↗

Translation Initiation Fidelity Defective Mutations in eIF5 and eIF2β Show Distinct Sensitivity to the Sequence Context for Recognition of the UUG Start Codon.Semantic proximity Read ↗

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