Article
Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.
Applied and environmental microbiology - 1 Mar 2008
Gursinsky Torsten, Gröbe Daniel, Schierhorn Angelika, Jäger Jana, Andreesen Jan R, Söhling Brigitte
Abstract excerpt
Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of selenocysteine into the nascent polypeptide chain. The synthesis of three different selenoproteins...
Topics
- Bacterial Proteins
- Base Pairing
- Base Sequence
- Blotting, Western
- Codon, Terminator
- Escherichia coli
- Eubacterium
- Mass Spectrometry
- Molecular Sequence Data
- Mutation
- RNA, Messenger
- RNA, Transfer, Amino Acid-Specific
- Selenocysteine