Article
RNA stem-loop enhanced expression of previously non-expressible genes.
Nucleic acids research - 26 May 2004
Paulus Michael, Haslbeck Martin, Watzele Manfred
Abstract excerpt
The key step in bacterial translation is formation of the pre-initiation complex. This requires initial contacts between mRNA, fMet-tRNA and the 30S subunit of the ribosome, steps that limit the initiation of translation. Here we report a method for improving translational initiation, which allows expression of several previously non-expressible genes. This method has potential applications in heterologous...
Topics
- Base Sequence
- Binding Sites
- Gene Expression Regulation
- Genetic Engineering
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Peptide Chain Initiation, Translational
- Protein Biosynthesis
- Proteins
- RNA Stability
- RNA, Messenger
- Regulatory Sequences, Ribonucleic Acid
- Ribonuclease H
- Ribosomes
- Transcription, Genetic