Article
RNA stem-loop enhanced expression of previously non-expressible genes.
Nucleic acids research - 26 May 2004
Paulus Michael, Haslbeck Martin, Watzele Manfred
Abstract excerpt
The key step in bacterial translation is formation of the pre-initiation complex. This requires initial contacts between mRNA, fMet-tRNA and the 30S subunit of the ribosome, steps that limit the initiation of translation. Here we report a method for improving translational initiation, which allows expression of several previously non-expressible genes. This method has potential applications in heterologous...
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