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Article

Optimized allele-specific real-time PCR assays for the detection of common mutations in KRAS and BRAF.

The Journal of molecular diagnostics : JMD - 1 Jan 2011

Lang Alois H, Drexel Heinz, Geller-Rhomberg Simone, Stark Nicole, Winder Thomas, Geiger Kathrin, Muendlein Axel

Abstract excerpt

Mutations in the oncogenes KRAS and BRAF have been identified as prognostic factors in patients with colorectal diseases and as predictors of negative outcome in epidermal growth factor receptor-targeted therapies. Therefore, accurate mutation detection in both genes, KRAS and BRAF, is of increasing clinical relevance. We aimed at optimizing allele-specific real-time PCR assays for the detection of common...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Open in PubMed Open full text

Identifiers

PMID: 21227391
DOI: 10.1016/j.jmoldx.2010.11.007
PMCID: PMC3070579

Genes

BRAF
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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