Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients.

doi:10.1371/journal.pone.0147672

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Article

Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients.

PloS one - 1 Jan 2016

Seekhuntod Sirirat, Thavarungkul Paninee, Chaichanawongsaroj Nuntaree

Abstract excerpt

BACKGROUND: Patients with KRAS mutations do not respond to epidermal growth factor receptor (EGFR) inhibitors and fail to benefit from adjuvant chemotherapy. Mutation analysis of KRAS is needed before starting treatment with monoclonal anti-EGFR antibodies in patients with metastatic colorectal cancer (mCRC). The objective of this study is to develop a multiplex allele-specific PCR (MAS-PCR) assay to detect KRAS...

Topics

Aged
Alleles
Amino Acid Sequence
Codon
Colorectal Neoplasms
DNA, Neoplasm
Female
Formaldehyde
Humans
Middle Aged
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Multiplex Polymerase Chain Reaction

Paraffin Embedding

Point Mutation

Reproducibility of Results

Sequence Analysis, DNA

ras Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 26812617
DOI: 10.1371/journal.pone.0147672
PMCID: PMC4728071

Genes

KRAS
EGFR

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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