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Article

Asymmetric mutant-enriched polymerase chain reaction and quantitative DNA melting analysis of KRAS mutation in colorectal cancer.

Analytical biochemistry - 1 Feb 2020

Botezatu Irina V, Kondratova Valentina N, Shelepov Valery P, Mazurenko Natalia N, Tsyganova Irina V, Susova Olga Y, Lichtenstein Anatoly V

Abstract excerpt

Identification of mutant genes in tumor tissues and blood plasma (solid and liquid biopsy samples, respectively) is a necessity for individualized treatment of cancer patients. Here we report the use of a novel mutant-enriched PCR - quantitative DNA melting curve analysis (mePCR-qDMA) with TaqMan probes. The TaqMan probes served as blocking agents during PCR and as hybridization probes during DNA melting curve...

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PMID: 31782995
DOI: 10.1016/j.ab.2019.113517

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