Article
Sensitive detection of trace amounts of KRAS codon 12 mutations by a fast and novel one-step technique.
Clinical biochemistry - 1 Nov 2014
Xie Feifei, Huang Jie, Qu Shoufang, Wu Weili, Jiang Jun, Wang Huagui, Wang Shujuan, Liu Qi, Zhang Senlin, Xu Lizhi, Gao Shangxian, Zhang Yunqing, Zhao Jinyin, Chen Weijun
Abstract excerpt
OBJECTIVES: The objective of this study is to develop a novel and sensitive method for KRAS codon 12 mutation testing. DESIGN AND METHODS: We developed a sensitive one-step real-time digestion-and-block TaqMan probe PCR (RTDB-PCR) technique that uses a thermostable endonuclease and a minor groove binder (MGB) blocker to detect KRAS codon 12 mutations. Dilution mimic DNA panels were used to assess the sensitivity...
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