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Article

Enhanced asymmetric blocked qPCR method for affordable detection of point mutations in KRAS oncogene.

Analytical and bioanalytical chemistry - 1 May 2021

Lázaro Ana, Tortajada-Genaro Luis A, Maquieira Ángel

Abstract excerpt

An accurate genetic diagnostic is key for adequate patient management and the suitability of healthcare systems. The scientific challenge lies in developing methods to discriminate those patients with certain genetic variations present in tumor cells at low concentrations. We report a method called enhanced asymmetric blocked qPCR (EAB-qPCR) that promotes the blocker annealing against the primer-template hybrid...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 33619642
DOI: 10.1007/s00216-021-03229-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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