Article
Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing.
Biochemical and biophysical research communications - 18 Feb 2011
Bontems Franck, Baerlocher Loic, Mehenni Sabrina, Bahechar Ilham, Farinelli Laurent, Dosch Roland
Abstract excerpt
Fish models like medaka, stickleback or zebrafish provide a valuable resource to study vertebrate genes. However, finding genetic variants e.g. mutations in the genome is still arduous. Here we used a combination of microarray capturing and next generation sequencing to identify the affected gene in the mozartkugelp11cv (mzlp11cv) mutant zebrafish. We discovered a 31-bp deletion in macf1 demonstrating the...
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