Article
A rapid and efficient method of genotyping zebrafish mutants.
Developmental dynamics : an official publication of the American Association of Anatomists - 1 Dec 2009
Parant John M, George Stephen A, Pryor Rob, Wittwer Carl T, Yost H Joseph
Abstract excerpt
In order to facilitate high throughput genotyping of zebrafish, we have developed a novel technique that uses High Resolution Melting Analysis (HRMA) to distinguish wild-type, heterozygous mutants and homogyzous mutants. This one hour technique removes the need for restriction enzymes and agarose gels. The generated melting curve profiles are sensitive enough to detect non-specific PCR products. We have been able...
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