Article
Making temperature-sensitive mutants.
Methods in enzymology - 1 Jan 2010
Ben-Aroya Shay, Pan Xuewen, Boeke Jef D, Hieter Philip
Abstract excerpt
The study of temperature-sensitive (Ts) mutant phenotypes is fundamental to gene identification and for dissecting essential gene function. In this chapter, we describe two "shuffling" methods for producing Ts mutants using a combination of PCR, in vivo recombination, and transformation of diploid strains heterozygous for a knockout of the desired mutation. The main difference between the two methods is the type...
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