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Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Protacio Reine U, Wahls Wayne P

Abstract excerpt

Precise allele replacement by homologous recombination (also known as "gene targeting" or "genome editing") allows scientists to engineer altered DNA sequences, insertions, or deletions at specific locations in the genome. Such reverse genetics provides powerful tools to elucidate the structure and function of regulatory DNA elements, genes, RNAs, and proteins within their natural, endogenous context. Here, we...

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PMID: 39527204
DOI: 10.1007/978-1-0716-4168-2_16
PMCID: PMC11694354

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