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Article

Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae.

Molecular cell - 25 Apr 2008

Ben-Aroya Shay, Coombes Candice, Kwok Teresa, O'Donnell Kathryn A, Boeke Jef D, Hieter Philip

Abstract excerpt

The Saccharomyces cerevisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic...

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Identifiers

PMID: 18439903
DOI: 10.1016/j.molcel.2008.02.021
PMCID: PMC4130347

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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