Article
PCR mutagenesis and gap repair in yeast.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Weir Mark, Keeney Jill B
Abstract excerpt
Random mutagenesis of a gene and subsequent screening for phenotypic properties is a basic genetic procedure for studying gene function. When working with yeast, cloning mutated alleles into a yeast vector can be done directly due to the efficiency of homologous recombination. Here we give sample protocols for introducing random mutations to a gene using PCR and transformation of the resulting product into yeast....
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