Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A high-efficiency method to replace essential genes with mutant alleles in yeast.

Yeast (Chichester, England) - 30 Jul 2005

Widlund Per O, Davis Trisha N

Abstract excerpt

Temperature-sensitive (TS), internally deleted and truncated alleles are important tools to facilitate the characterization of essential genes. We have developed a straightforward method to replace a wild-type gene with a mutant allele at the endogenous locus. This method is an efficient alternative to the two-step method for integration of alleles that are compromised in function or contain multiple mutations. A...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Fungal Proteins
Share to Gene Targeting
Share to Genes, Essential

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genes, Fungal

Share to Hydroxymethyl and Formyl Transferases

Share to Orotic Acid

Share to Temperature

Share to Yeasts

Source

Open in PubMed Open full text

Identifiers

PMID: 16088871
DOI: 10.1002/yea.1244
PMCID: PMC1698466

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A high-efficiency method to replace essential genes with mutant alleles in yeast.PMID 16088871Read ↗

Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae.Semantic proximity Read ↗

Efficient targeted mutation of genomic essential genes in yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

Study Essential Gene Functions by Plasmid Shuffling.Semantic proximity Read ↗

An Updated Collection of Sequence Barcoded Temperature-Sensitive Alleles of Yeast Essential Genes.Semantic proximity Read ↗

Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system.Semantic proximity Read ↗

Exploring conditional gene essentiality through systems genetics approaches in yeast.Semantic proximity Read ↗

Systematic exploration of essential yeast gene function with temperature-sensitive mutants.Semantic proximity Read ↗

Exploration of essential gene functions via titratable promoter alleles.Semantic proximity Read ↗

An easy and fast alternative to plasmid shuffling for the identification of in vitro mutagenized alleles of essential genes of Saccharomyces cerevisiae.Semantic proximity Read ↗

Approaches to Study Proteins Encoded by Essential Genes.Semantic proximity Read ↗

Two-step method for constructing unmarked insertions, deletions and allele substitutions in the yeast genome.Semantic proximity Read ↗

A recombinatorial method useful for cloning dominant alleles in Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.