Systematic exploration of essential yeast gene function with temperature-sensitive mutants.

doi:10.1038/nbt.1832

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Article

Systematic exploration of essential yeast gene function with temperature-sensitive mutants.

Nature biotechnology - 1 Apr 2011

Li Zhijian, Vizeacoumar Franco J, Bahr Sondra, Li Jingjing, Warringer Jonas, Vizeacoumar Frederick S, Min Renqiang, Vandersluis Benjamin, Bellay Jeremy, Devit Michael, Fleming James A, Stephens Andrew, Haase Julian, Lin Zhen-Yuan, Baryshnikova Anastasia, Lu Hong, Yan Zhun, Jin Ke, Barker Sarah, Datti Alessandro, Giaever Guri, Nislow Corey, Bulawa Chris, Myers Chad L, Costanzo Michael, Gingras Anne-Claude, Zhang Zhaolei, Blomberg Anders, Bloom Kerry, Andrews Brenda, Boone Charles

Abstract excerpt

Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (∼45%) of the 1,101 essential yeast genes, with ∼30% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the S288C common reference strain and are linked to a kanMX...

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PMID: 21441928
DOI: 10.1038/nbt.1832
PMCID: PMC3286520

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