Article
COLD PCR HRM: a highly sensitive detection method for IDH1 mutations.
Human mutation - 1 Dec 2010
Boisselier Blandine, Marie Yannick, Labussière Marianne, Ciccarino Pietro, Desestret Virginie, Wang XiaoWei, Capelle Laurent, Delattre Jean-Yves, Sanson Marc
Abstract excerpt
The p.Arg132His mutation of isocitrate dehydrogenase 1 (IDH1(R132H) ) is a frequent alteration and a major prognostic marker in gliomas. However, direct sequencing of highly contaminated tumor samples may fail to detect this mutation. Our objective was to evaluated the sensitivity of a newly described amplification method, coamplification at lower temperature-PCR (COLD PCR), combined with high-resolution melting...
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