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Article

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.

Nature medicine - 1 May 2008

Li Jin, Wang Lilin, Mamon Harvey, Kulke Matthew H, Berbeco Ross, Makrigiorgos G Mike

Abstract excerpt

PCR is widely employed as the initial DNA amplification step for genetic testing. However, a key limitation of PCR-based methods is the inability to selectively amplify low levels of mutations in a wild-type background. As a result, downstream assays are limited in their ability to identify subtle genetic changes that can have a profound impact in clinical decision-making and outcome. Here we describe...

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PMID: 18408729
DOI: 10.1038/nm1708

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KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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