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Article

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.

Biochemical Society transactions - 1 Apr 2009

Li Jin, Makrigiorgos G Mike

Abstract excerpt

PCR is widely employed as the initial DNA amplification step for genetic testing and cancer biomarker detection. However, a key limitation of PCR-based methods, including real-time PCR, is the inability to selectively amplify low levels of variant alleles in a wild-type allele background. As a result, downstream assays are limited in their ability to identify subtle genetic changes that can have a profound impact...

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PMID: 19290875
DOI: 10.1042/BST0370427

Genes

EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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