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Article

Clinical application of a highly sensitive digital PCR assay to detect a small fraction of IDH1 R132H-mutant alleles in diffuse gliomas.

Brain tumor pathology - 1 Oct 2022

Satomi Kaishi, Yoshida Akihiko, Matsushita Yuko, Sugino Hirokazu, Fujimoto Kenji, Honda-Kitahara Mai, Takahashi Masamichi, Ohno Makoto, Miyakita Yasuji, Narita Yoshitaka, Yatabe Yasushi, Shibahara Junji, Ichimura Koichi

Abstract excerpt

The current World Health Organization classification of diffuse astrocytic and oligodendroglial tumors requires the examination of isocitrate dehydrogenase 1 (IDH1) or IDH2 mutations. Conventional analysis tools, including Sanger DNA sequencing or pyrosequencing, fail in detecting these variants of low frequency owing to their limited sensitivity. Digital polymerase chain reaction (dPCR) is a recently developed,...

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PMID: 35902443
DOI: 10.1007/s10014-022-00442-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Clinical application of a highly sensitive digital PCR assay to detect a small fraction of IDH1 R132H-mutant alleles in diffuse gliomas.PMID 35902443Read ↗

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