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Article

COLD-PCR enrichment of rare cancer mutations prior to targeted amplicon resequencing.

Clinical chemistry - 1 Mar 2012

Milbury Coren A, Correll Mick, Quackenbush John, Rubio Renee, Makrigiorgos G Mike

Abstract excerpt

BACKGROUND: Despite widespread interest in next-generation sequencing (NGS), the adoption of personalized clinical genomics and mutation profiling of cancer specimens is lagging, in part because of technical limitations. Tumors are genetically heterogeneous and often contain normal/stromal cells, features that lead to low-abundance somatic mutations that generate ambiguous results or reside below NGS detection...

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Identifiers

PMID: 22194627
DOI: 10.1373/clinchem.2011.176198
PMCID: PMC3418918

Genes

EGFR
IDH1
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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