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Article

COLD-PCR: improving the sensitivity of molecular diagnostics assays.

Expert review of molecular diagnostics - 1 Mar 2011

Milbury Coren A, Li Jin, Liu Pingfang, Makrigiorgos G Mike

Abstract excerpt

The detection of low-abundance DNA variants or mutations is of particular interest to medical diagnostics, individualized patient treatment and cancer prognosis; however, detection sensitivity for low-abundance variants is a pronounced limitation of most currently available molecular assays. We have recently developed coamplification at lower denaturation temperature-PCR (COLD-PCR) to resolve this limitation....

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Identifiers

PMID: 21405967
DOI: 10.1586/erm.10.115
PMCID: PMC3111913

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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