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Article

Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.

Nucleic acids research - 1 Jan 2011

Milbury Coren A, Li Jin, Makrigiorgos G Mike

Abstract excerpt

Identifying low-abundance mutations within wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. However, utilizing the clinical and diagnostic potential of rare mutations is limited by sensitivity of the molecular techniques employed, especially when the type and position of mutations are unknown. We have developed a novel platform that...

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Identifiers

PMID: 20937629
DOI: 10.1093/nar/gkq899
PMCID: PMC3017621

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