Article
Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.
The Journal of molecular diagnostics : JMD - 1 Mar 2011
Milbury Coren A, Chen Clark C, Mamon Harvey, Liu Pingfang, Santagata Sandro, Makrigiorgos G Mike
Abstract excerpt
Thorough screening of cancer-specific biomarkers, such as DNA mutations, can require large amounts of genomic material; however, the amount of genomic material obtained from some specimens (such as biopsies, fine-needle aspirations, circulating-DNA or tumor cells, and histological slides) may limit the analyses that can be performed. Furthermore, mutant alleles may be at low-abundance relative to wild-type DNA,...
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