Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Insight into the mechanism of the stabilization of moloney murine leukaemia virus reverse transcriptase by eliminating RNase H activity.

Bioscience, biotechnology, and biochemistry - 1 Jan 2010

Mizuno Masaki, Yasukawa Kiyoshi, Inouye Kuniyo

Abstract excerpt

We explored the mechanism of the stabilization of Moloney murine leukaemia virus reverse transcriptase (MMLV RT) by eliminating RNase H activity. Without the template-primer (T/P) poly(rA)-p(dT)(15), the temperature reducing initial reverse-transcription activity by 50% over a 10-min incubation of the RNase H activity-deficient variant D524A was higher by 3.7 degrees C than that of the wild-type enzyme (WT). In...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Enzyme Stability
Share to Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Moloney murine leukemia virus

Share to Mutation

Share to Reverse Transcription

Share to Ribonuclease H

Share to RNA-Directed DNA Polymerase

Share to Temperature

Source

Identifiers

PMID: 20139597
DOI: 10.1271/bbb.90777

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Insight into the mechanism of the stabilization of moloney murine leukaemia virus reverse transcriptase by eliminating RNase H activity.PMID 20139597Read ↗

The role of template-primer in protection of reverse transcriptase from thermal inactivation.Semantic proximity Read ↗

Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis.Semantic proximity Read ↗

Stabilization of human immunodeficiency virus type 1 reverse transcriptase by site-directed mutagenesis.Semantic proximity Read ↗

Stabilization of Moloney murine leukemia virus reverse transcriptase by site-directed mutagenesis of surface residue Val433.Semantic proximity Read ↗

Reversion of a Moloney murine leukemia virus RNase H mutant at a second site restores enzyme function and infectivity.Semantic proximity Read ↗

Thermal effects on reverse transcription: improvement of accuracy and processivity in cDNA synthesis.Semantic proximity Read ↗

Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase.Semantic proximity Read ↗

Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.Semantic proximity Read ↗

Mutations within the primer grip region of HIV-1 reverse transcriptase result in loss of RNase H function.Semantic proximity Read ↗

Metal binding and activation of the ribonuclease H domain from moloney murine leukemia virus.Semantic proximity Read ↗

Analysis of the RNA- and DNA-dependent DNA polymerase activities of point mutants of HIV-1 reverse transcriptase lacking ribonuclease H activity.Semantic proximity Read ↗

The effect of template RNA structure on elongation by HIV-1 reverse transcriptase.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.