Article
Mutations within the primer grip region of HIV-1 reverse transcriptase result in loss of RNase H function.
The Journal of biological chemistry - 25 Apr 1997
Palaniappan C, Wisniewski M, Jacques P S, Le Grice S F, Fay P J, Bambara R A
Abstract excerpt
Human immunodeficiency virus (HIV) DNA synthesis is accompanied by degradation of genomic RNA by the RNase H of reverse transcriptase (RT). Two different modes of RNase H activity appear necessary for complete RNA removal. In one, occurring during minus strand synthesis, positioning of the RNase...
Topics
- Binding Sites
- HIV Reverse Transcriptase
- HIV-1
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- RNA, Viral
- Ribonuclease H
- Structure-Activity Relationship
- Substrate Specificity