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Article

Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis.

Journal of biotechnology - 1 Nov 2010

Yasukawa Kiyoshi, Mizuno Masaki, Konishi Atsushi, Inouye Kuniyo

Abstract excerpt

We hypothesized that the thermal stability of Moloney murine leukaemia virus reverse transcriptase (MMLV RT) will increase with increases in its ability to bind with a template-primer (T/P). To test this hypothesis, we introduced positive charges into MMLV RT by site-directed mutagenesis at positions that have been implicated in the interaction with T/P. Thirty-six variants were constructed in which one of the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 20933548
DOI: 10.1016/j.jbiotec.2010.09.961

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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