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Article

Stabilization of human immunodeficiency virus type 1 reverse transcriptase by site-directed mutagenesis.

Biotechnology letters - 1 Dec 2013

Nishimura Kosaku, Shinomura Mayu, Konishi Atsushi, Yasukawa Kiyoshi

Abstract excerpt

Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) is a heterodimer containing 66 kDa p66 and 51 kDa p51 subunits. We previously showed that HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT have higher affinities for dTTP and template-primer (T/P) than Moloney murine leukemia virus RT, which is currently used for cDNA synthesis, suggesting that they might also be useful for cDNA...

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Share to Electrophoresis, Polyacrylamide Gel
Share to Enzyme Stability

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to HIV Reverse Transcriptase

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Share to Mutagenesis, Site-Directed

Share to RNA, Viral

Share to Sequence Analysis, DNA

Source

Identifiers

PMID: 24078120
DOI: 10.1007/s10529-013-1321-4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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