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Article

The role of template-primer in protection of reverse transcriptase from thermal inactivation.

Nucleic acids research - 15 Jul 2002

Gerard Gary F, Potter R Jason, Smith Michael D, Rosenthal Kim, Dhariwal Gulshan, Lee Jun, Chatterjee Deb K

Abstract excerpt

We compared the thermal stabilities of wild-type recombinant avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) with those of mutants of the recombinant enzymes lacking RNase H activity. They differed in resistance to thermal inactivation at elevated temperatures in the presence of an RNA/DNA template-primer. RNase H-minus RTs retained the ability to efficiently...

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Identifiers

PMID: 12136094
DOI: 10.1093/nar/gkf417
PMCID: PMC135738

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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