Article
Thermal effects on reverse transcription: improvement of accuracy and processivity in cDNA synthesis.
BioTechniques - 1 May 2001
Malboeuf C M, Isaacs S J, Tran N H, Kim B
Abstract excerpt
Reverse transcription, coupled with DNA amplification, has been widely used for molecular analysis of RNAs. Reverse transcriptases are retroviral DNA polymerases that can synthesize DNA from both RNA and DNA. In general, because of the lack of 3'-->5' exonuclease activity in retroviral reverse transcriptases, the reverse transcription step is error prone. Mutations created during the reverse transcription step of...
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