Article
Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.
Bioscience, biotechnology, and biochemistry - 1 Dec 2017
Katano Yuta, Li Tongyang, Baba Misato, Nakamura Miyo, Ito Masaaki, Kojima Kenji, Takita Teisuke, Yasukawa Kiyoshi
Abstract excerpt
We attempted to increase the thermostability of Moloney murine leukemia virus (MMLV) reverse transcriptase (RT). The eight-site saturation mutagenesis libraries corresponding to Ala70-Arg469 in the whole MMLV RT (Thr24-Leu671), in each of which 1 out of 50 amino acid residues was replaced with other amino acid residue, were constructed. Seven-hundred and sixty eight MMLV RT clones were expressed using a cell-free...
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