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Article

Rational design of HIV-1 fluorescent hydrolysis probes considering phylogenetic variation and probe performance.

Journal of virological methods - 1 May 2010

Althaus Claudia F, Gianella Sara, Rieder Philip, von Wyl Viktor, Kouyos Roger D, Niederöst Barbara, Schmid Adrian, Metzner Karin J, Joos Beda, Günthard Huldrych F, Fischer Marek

Abstract excerpt

Quantitative PCR (qPCR) using fluorescent hydrolysis probes (FH-probes; TaqMan-probes) of variable genomes, such as HIV-1, can result in underestimation of viral copy numbers due to mismatches in the FH-probe's target sequences. Therefore both target conservation and physical properties of FH-pro...

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Source

Identifiers

PMID: 20116399
DOI: 10.1016/j.jviromet.2010.01.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-20116399-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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