Article
Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR.
Nucleic acids research - 1 Jan 2007
Huang Shihai, Salituro John, Tang Ning, Luk Ka-Cheung, Hackett John, Swanson Priscilla, Cloherty Gavin, Mak Wai-Bing, Robinson John, Abravaya Klara
Abstract excerpt
Real-time PCR assays have recently been developed for diagnostic and research purposes. Signal generation in real-time PCR is achieved with probe designs that usually depend on exonuclease activity of DNA polymerase (e.g. TaqMan probe) or oligonucleotide hybridization (e.g. molecular beacon). Probe design often needs to be specifically tailored either to tolerate or to differentiate between sequence variations....
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