Article
Plug-and-Play Competitive Hairpin Conversion Module Enables Enzyme-Free High-Resolution Discrimination of Single Nucleotide Polymorphisms.
Analytical chemistry - 28 Oct 2025
Yu Siyu, Zhang Yunshan, Yang Fang, Wang Xueshuai, Liu Xiao, Wang Zhou, Ye Jing, Chen Jian, Feng Xianzhong, Zhang Diming
Abstract excerpt
The accuracy of single-nucleotide polymorphism (SNP) detection in long sequences is fundamentally constrained by the minuscule thermodynamic differences arising from single-base mismatches, wherein the overall probe binding energy frequently masks single-base-discrimination signals. Here, we develop a plug-and-play competitive hairpin conversion module (CHCM) based on strand-competitive hybridization and...
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