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Multiplex qPCR Assay for HIV-1 Proviral DNA Detection and Subtype Characterization: Exploiting Quenching of Multiple Fluorophores with a Single Quencher Operating in<i>Trans</i>

2025-03-14

Abstract excerpt

Accurate detection and quantification of HIV-1 proviral DNA are critical for effective patient monitoring and therapeutic decision-making. In this study, we developed a multiplexed quantitative PCR (qPCR) assay designed to detect HIV-1 proviral DNA, determine viral subtype, specifically identifying the predominant subtype C and validate assay performance using an internal control. Gene-specific primers were engine...

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Literature Corpus work
469993ce-2483-52d3-9c74-f719f216550d
DOI
10.1101/2025.03.13.25323928
Open publication

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Multiplex qPCR Assay for HIV-1 Proviral DNA Detection and Subtype Characterization: Exploiting Quenching of Multiple Fluorophores with a Single Quencher Operating in<i>Trans</i>DOI 10.1101/2025.03.13.25323928
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