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Article

PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens.

Communications biology - 18 Feb 2021

MacLeod Iain J, Rowley Christopher F, Essex M

Abstract excerpt

Sensitive and reproducible diagnostics are fundamental to containing the spread of existing and emerging pathogens. Despite the reliance of clinical virology on qPCR, technical challenges persist that compromise their reliability for sustainable epidemic containment as sequence instability in probe-binding regions produces false-negative results. We systematically violated canonical qPCR design principles to...

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Identifiers

PMID: 33603155
DOI: 10.1038/s42003-021-01751-9
PMCID: PMC7892852

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