Article

Scaling Variant-Aware Multiplex Primer Design

2026-02-06

Abstract excerpt

<h4>Motivation</h4> Robust primer design is essential for reliable multiplex PCR in diverse and evolving pathogen, microbial, and host genomes. Traditional methods optimized for a single reference often fail on emerging variants, leading to reduced efficiency. Variant-aware design seeks primers that remain effective across diverse targets, but this introduces two key challenges: identifying robust candidates and...

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Literature Corpus work: 00de997b-47dd-5965-a589-141dece20007
DOI: 10.64898/2026.02.03.703607

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