Article

In silico thermodynamic evaluation of the effectiveness of RT-LAMP primers to SARS-CoV-2 variants detection

2023-08-09

Abstract excerpt

Viral mutations are the primary cause of mismatches in primer-target hybridisation, affecting the sensibility of molecular techniques, potentially leading to detection dropouts. Despite its importance, little is known about the quantitative effect of mismatches in primer-target hybridisation. We use up-to-date and highly detailed thermodynamic model parameters of DNA mismatches to evaluate the sensibility to varia...

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Literature Corpus work: 02b31058-38f3-5e8b-b049-cc03fb0347a7
DOI: 10.1101/2023.08.08.552530

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